Table 3. Summary of in situ and immunohistochemistry replicates for uninfected embryos.

lists the number of replicates performed for each gene at each age. For each embryo used in this study, adjacent sections were taken onto three alternating slides.

Gene	HH 28, E5	HH 29, E6	HH 33, E8	HH 36, E10
Sema3F	4	4	3	3
Sema3D	5	5	4	4
Nrp1	4	4	6	3
Nrp2	5	4	4	3
PlxnA1	3	6	4	3

With adjacent sections on three sets of slides, three different probes and/or antibodies could be used on the same embryo. Therefore, one embryo may serve as a replicate for up to three labels for any given age. Abbreviations: E, embryonic day; HH, Hamburger Hamilton stage