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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Physiol Behav. 2017 Aug 10;203:100–112. doi: 10.1016/j.physbeh.2017.08.002

Table 2.

Summary of the modified UCMS procedures employed in Experiment 3. Changes from the original protocol are designated with an asterisk (*).

Day Time Procedure
1 O/N Exposure to damp bedding
2 AM 1-h immobilization in restraint tube
3 AM* 5-min exposure to 2% isoflurane anesthesia
O/N Cage tilt
4 AM 1-h exposure to soiled rat bedding
AM* 5-min forced swim
5 All day Exposure to damp bedding
O/N Food and water deprivation
6 AM 5-min forced swim
PM 12-h housing under shifted light cycle
7 PM 1-h immobilization in restraint tube
O/N Individual/isolation housing
8 AM 5-min exposure to 2% isoflurane anesthesia
PM 1-h exposure to brightly lit inescapable arena
O/N Housing with bedding from strange males
9 AM 1-h immobilization in restraint tube
PM 1-h exposure to soiled rat bedding
O/N Food and water deprivation
10 AM* 15-min forced swim
O/N Cage tilt
11 AM* 3-h housing under shifted light cycle
PM* 1-h exposure to bobcat urine
O/N Housing with bedding from strange males