Table 2.
Summary of the modified UCMS procedures employed in Experiment 3. Changes from the original protocol are designated with an asterisk (*).
| Day | Time | Procedure |
|---|---|---|
| 1 | O/N | Exposure to damp bedding |
| 2 | AM | 1-h immobilization in restraint tube |
| 3 | AM* | 5-min exposure to 2% isoflurane anesthesia |
| O/N | Cage tilt | |
| 4 | AM | 1-h exposure to soiled rat bedding |
| AM* | 5-min forced swim | |
| 5 | All day | Exposure to damp bedding |
| O/N | Food and water deprivation | |
| 6 | AM | 5-min forced swim |
| PM | 12-h housing under shifted light cycle | |
| 7 | PM | 1-h immobilization in restraint tube |
| O/N | Individual/isolation housing | |
| 8 | AM | 5-min exposure to 2% isoflurane anesthesia |
| PM | 1-h exposure to brightly lit inescapable arena | |
| O/N | Housing with bedding from strange males | |
| 9 | AM | 1-h immobilization in restraint tube |
| PM | 1-h exposure to soiled rat bedding | |
| O/N | Food and water deprivation | |
| 10 | AM* | 15-min forced swim |
| O/N | Cage tilt | |
| 11 | AM* | 3-h housing under shifted light cycle |
| PM* | 1-h exposure to bobcat urine | |
| O/N | Housing with bedding from strange males |