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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Sep 5;143(4):1560–1574.e6. doi: 10.1016/j.jaci.2018.08.023

Figure 7.

Figure 7.

Lef1 regulates MSC differentiation. A, Transfection efficacy of Lef1 shRNA was confirmed using flow cytometry analysis. B-C, A dose-dependent deletion of Lef1 was detected by RT-PCR (B) and western blot (C). D, Immunofluorescence staining of MSCs transfected with control shRNA and Lef1 shRNA with the primary antibody against α-SMA. E, Representative immunoblots for α-SMA in MSCs transfected with control shRNA and Lef1 shRNA. F, Immunofluorescence staining of MSCs transfected with control shRNA and Lef1 shRNA with the primary antibody against E-cadherin. G, Representative immunoblots for E-Cadherin in MSCs transfected with control shRNA and Lef1 shRNA. Data represent mean ± SEMs of three independent experiments. *P<0.05, **P<0.01.