TABLE E2.
Antibodies used for flow cytometry and immunohistochemical studies
| Antibody | Clone | Species | Application | Source |
|---|---|---|---|---|
| CD11b | M1/70 | Rat | FC (0.25 µg/105cells) | BioLegend |
| CD29 | HMβ1–1 | Hamster | FC (0.25 µg/105cells) | BioLegend |
| CD31 | 390 | Rat | FC (0.25 µg/105cells) | BioLegend |
| CD34 | HM34 | Hamster | FC (0.25 µg/105cells) | BioLegend |
| Collagen 1 | NB600–408 | Rabbit | WB (1:1000) | Novus Biologicals |
| E-cadherin | 24E10 | Rabbit | IHC (1:200) ICC (1:200) WB (1:1000) |
Cell Signaling Technology |
| Epi-CAM | G8.8 | Rat | IHC (1:100) | ThermoFisher |
| EYFP | GF28R | Mouse | IHC (1:100) | ThermoFisher |
| NB600–303 | Rabbit | IHC (1:100) | Novus Biologicals | |
| Lef1 | C12A5 | Rabbit | IHC (1:200) | Cell Signaling Technology |
| LepR | BAF497 | Goat | FC (1:50) | R&D Systems |
| Nestin | 1D11H2 | Mouse | FC (1:100) | Proteintech |
| pLef1 | SAB4504256 | Rabbit | WB (1:1000) | Sigma-Aldrich |
| RhoA- GTPase |
26904 | Mouse | IHC (1:100) ICC (1:50) WB (1:500) |
New East Biosciences |
| Sca1 | D7 | Rat | FC (0.25 µg/105cells) | BioLegend |
| TER-119 | BAM1125 | Rat | FC (1:50) | R&D Systems |
| Vimetin | V9 | Mouse | IHC (1:50) | ThermoFisher |
| α-SMA | SP171 | Rabbit | IHC (1:100) ICC (1:100) WB (1:1000) |
Sigma-Aldrich |
IHC, Immunohistochemistry; ICC, Immunocytochemistry; WB, Western blotting; FC, Flow cytrometry