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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2018 Sep 5;14(2):173–187. doi: 10.1007/s11481-018-9808-3

Figure 2. Increase in TH and dopamine in differentiated mouse MN9D neuronal cells by aspirin.

Figure 2.

(A) Mouse MN9D neuronal cells were allowed to differentiate in neurobasal media containing B27 for 2 d following treatment with 2 μM aspirin for different time periods in neurobasal media without B27. The protein level of TH was monitored by Western blot (A). Bands were scanned and values (TH/actin) presented as relative to control (B). Results are mean SD of different experiments. *p < 0.05 & ***p < 0.001 vs control; ns, not significant. Cells were treated with 2 µM aspirin for 2 h followed by double-label immunofluorescence with antibodies against TH and actin (C). Mean fluorescence intensity (MFI) of TH and actin was calculated in 20 different cells (D) and presented as MFI-TH/MFI-actin. Results are mean ± SEM of 20 different cells per group. ***p < 0.001 vs control; ns, not significant. E) Cells were treated with 2 µM aspirin for different time periods followed by measuring the level of DA, DOPAC and HVA in supernatants by HPLC. Results are mean ± SD of at least three independent experiments. *p < 0.05 & ***p < 0.001 vs control; ns, not significant. One-way ANOVA with Tukey’s multiple comparison test shows that time-dependent increase in DA by aspirin is significant (p < 0.0001).