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. 2019 Feb 27;10:317. doi: 10.3389/fimmu.2019.00317

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Copyright © 2019 Liu, Zhou, Wu, Hu, Yang, Li, Wu, Chen, Chen, Lin, Cui, Fu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunosuppressive microenvironment effects of H-RT and L-TBI combination therapy on 4T1 tumor-bearing mice. The percentage of the G-MDSCs (defined as CD45⁺CD11c⁻CD11b⁺Ly6G⁺Ly6c^low) (A), M-MDSCs (defined as CD45⁺CD11c⁻CD11b⁺Ly6G⁻Ly6c^hi) (B), M1 (defined as CD45⁺CD11b⁺F4/80⁺ CD206⁻) (C), M2 (defined as CD45⁺CD11b⁺ F4/80⁺CD206⁺) (D), and Eosinophils (defined as Siglec-F⁺Gr1^low) (E) were analyzed by flow cytometry analysis. Data are representative charts or the percentages of individual subjects. Data are expressed as mean ± SE of 5 mice/group. The statistical significance of differences was determined by ANOVA. (^*P < 0.05, ^**P < 0.01, ^***P < 0.001, and NS = not significant).