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. 2019 Mar 5;9:3477. doi: 10.1038/s41598-019-40242-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell proliferation on PCL and PCL-PNIPAAm surfaces. CCK-8 studies showed that both HDF cells and MSCs survived and proliferated on both PCL and PCL-P (PCL-PNIPAAm) using tissue culture plates as control (TCP CON) surfaces for 1, 3 and 7 days (a). At day 7, a significant increase in OD was observed in both cell types compared to day 1 and 3 and both types proliferated more on the surfaces of PCL-P than the surfaces of PCL (*p < 0.05; **p < 0.01; ***p < 0.001; ns: no significant difference). This trend of cell viability and proliferation was also observed in fluorescent microscopy for both HDF cells (b) and MSCs (c). HDF cells were stained with Live-dead staining (green: membrane of living cells, red: nuclei of dead cells) whereas GFP was cloned into MSCs. Emission was observed with fluorescent microscopy at days 3 and 7. Very dense and clustered cells with higher viability were observed at day 7 and on modified surfaces (PCL-P) than at day 3 and on non- modified PCL surfaces.