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. 2019 Mar 5;9:3477. doi: 10.1038/s41598-019-40242-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Trypsinization vs. reduced temperature comparison of cell detachment ratio and viability. Both HDF cells and MSCs in reduced temperature conditions (30 °C) showed significantly higher numbers of cell-detachment ratios from PCL-PNIPAAm surfaces than from PCL only surfaces. Trypsinization did not affect significantly the cell-detachment ratios between the surfaces but this technique allowed more detachment of cells from the surfaces of both PCL and PCL-PNPAAm surfaces. However, a higher cell viability rate was observed in the temperature dependent cell recovery technique than the trypsinization technique. Both HDF and MSCs had higher cell viability when recovered from PCL-PNIPAAm than trypsinization. *p < 0.05; **p < 0.01; ***p < 0.001; ns: no significant difference.