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. 2019 Mar 5;9:3477. doi: 10.1038/s41598-019-40242-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescent analysis. Representative Immunostaining results for ECM proteins of HDF cells. (a) Fibronectin, (b) Laminin and (c) Collagen I on cells detached from PCL-PNIPAAm macrocarriers by trypsin treatment and reduced temperature. Nuclei stained blue with Hoechst 33342 dye and proteins stained green with FITC-labelled secondary antibody. Immunofluorescent images showed that cells detached by reduced temperature contained fibronectin whereas no fibronectin was found in cells detached by trypsin treatment.