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. 2019 Mar 5;9:3442. doi: 10.1038/s41598-019-40231-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HFD-fed cytohesin-3 deficient mice have a normal metabolic rate. Male wt (white symbols; white and dotted bars) and cyth3^−/− mice (black symbols; hatched and black bars) received a high-fat diet (HFD, 60 kJ% fat) and control diet (CD, 13 kJ% fat), respectively. After 6 weeks mice were analyzed in metabolic cages for oxygen consumption (n = 7–8) (a) and carbon dioxide production (n = 7–8) (b). From these values energy expenditure (c) and the respiratory exchange ratio (d) were calculated. Body temperature was determined from HFD-fed mice (n = 4) (e) and Ucp1 in brown adipose tissue was analyzed by PCR. Activity (n = 7–8) (f) and food intake (n = 6–8) (g) were also determined in metabolic cages. Gene expression of Leptin in WATi and WATg was analyzed by PCR. The results are given in means or as individual data points ± SEM (*p < 0.05; **p < 0.01).