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. 2019 Mar 5;9:3442. doi: 10.1038/s41598-019-40231-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytohesin-3 deficient mice have an increased lipid excretion. Lipid excretion from wt (white symbols) and ko mice (black symbols) after HFD (n = 6–7) (a) and aged (in average 24 weeks old) wt and ko mice (wt, n = 3; ko, n = 5) (b) was analyzed by extraction from feces. Gene expression of Cyp7a1, Cyp7b1, Cyp8b1 and Cyp27a1 in livers from HFD- and CD-fed male wt (dotted and white bars) and ko mice (hatched and black bars) (Cyp7b1, n = 6–7; for all other Cyps n = 9–11) (c) and from two years old wt and cyth3^−/− male mice (n = 6) (d) was analyzed by PCR. The expression was normalized to Hprt. The results are given in means ± SEM (*p < 0.05; ***p < 0.001).