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. 2019 Jan 30;9(4):1096–1114. doi: 10.7150/thno.29673

Figure 5.

Figure 5

p53 and RAS mutants control the expression and intracellular localization of HDAC4 and HIF-1α. A. Selected images showing cellular co-localization of HDAC4 with HIF-1α. B. Selected images showing pHDAC4 was increased by p53 induction and ERK active RAS transfection. C. Detection of HIF-1α, HDAC4 and p-HDAC4 in the nucleus and cytoplasm of SKOV3T cells. β-Actin and histone 3 were used as loading controls for the cytoplasmic and nuclear extractions, respectively. D. Direct binding of HDAC4/p-HDAC4 with HIF-1α detected by co-immunoprecipitation in SKOV3T and SKOV3T/V12 cells. Rabbit or mouse IgG served as a negative control for the co-IP experiment. E-F. Detection of HDAC4, pHDAC4, HIF-1α, p53, RAS in additional ovarian cancer cell lines (E) and lung cancer cell lines (F) with either or both p53 or/and RAS mutations, showing that HDAC4, pHDAC4 and HIF-1α are regulated by p53 and RAS status. Protein markers are properly labeled in relative panels.