Skip to main content
. 2019 Jan 30;9(4):1181–1199. doi: 10.7150/thno.29093

Figure 8.

Figure 8

The lysosomotropic drug lapatinib stimulated CXCL5 expression in breast cancer cells. The fluorescence of the lysosomal probe (LysoTracker Red DND-99, Lyso) was evaluated by FACS analysis after 72 hours of treatment (5 μmol/L). (B) Cell extracts from control (C) or lapatinib (lapa, 5 μmol/L) 48-hour treated SKBR3 cells were tested for LAMP1 expression by immuno-blotting. Actin is shown as a loading control. (C) Determination of ROS production in cells treated with 5 μmol/L lapatinib for 48 hours. (D) CXCL5 mRNA levels were evaluated by qPCR in control (C), lapatinib (5 μmol/L) 48-hour treated SKBR3 cells. (E) CXCL5 protein levels were evaluated by ELISA in control (C) and lapatinib (5 μmol/L) 48-hour treated SKBR3 cells. P values are indicated; ** p < 0.01; *** p < 0.001.