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Confocal images of EGF-ATTO655-treated NCI-H460 (EGFR-negative) and A431 (EGFR-positive) cells. The cells were treated with free dye or EGF-ATTO655 for 5 h, and after washing the cells, fluorescence images of the cells were obtained. For competition assay, A431 cells were pre-incubated with excess unlabeled EGF (+ EGF group) and then treated with EGF-ATTO655. Yellow colored regions indicate localization of EGF-ATTO655 in lysosomal sites.
