Skip to main content
. 2019 Jan 30;9(4):1066–1084. doi: 10.7150/thno.29754

Figure 4.

Figure 4

Cold plasma increased antioxidants in skin tissues and cells. (A) The mRNA expression levels in ear skin tissues from plasma-treated and untreated (ctrl) mice were compared and summarized in the indicated experimental groups: SOD1, superoxide dismutase 1; CAT, catalase; TRX, thioredoxin; GSR, glutathione reductase; GPX2, glutathione peroxidase 2. (B) Western blot analysis of peroxireductase 6 (PRDX6), Sod1, catalase, thioredoxin reductase 1 (TRXR1), and GSR. (C) Keratinocytes were transfected with siRNA targeting NRF2 and evaluated for SOD1, SOD1, CAT, PRDX6, GSR, GCLC, GCLM, and Cx43 mRNA expression by qPCR. The mRNA level of scrambled siRNA and mocked control without siRNA were set to 1.14 (black lines) and 1 (dashed lines), respectively. The mRNA and protein level of untreated mice were arbitrarily set to 1 with n = 3 to 9 mice depending on time point (dashed lines in d and g). Males and females were used on d15 (as indicated); on d3/d6 only females were available for the measurements (n ≥ 3). The data are presented as mean +/- S.D.; *p<0.05, **p<0.01, ***p< 0.001, as compared to controls (ctrl) at each day and assay.