Table 1.

KD, app values of labeled and unlabeled 8708 and 8709 antibody fragment binding to A-431 cells

	Fragment	KD, app (unlabeled) (nM)	KD,app (IRDye800CW) (nM)
8708	Fab	ns	ns
	(scFv)2	110 ± 10	93 ± 3
	scFv-Fc	ns	ns
8709	Fab	6 ± 2	50 ± 2*
	(scFv)2	9 ± 3	18 ± 2
	scFv-Fc	12 ± 5	22 ± 6
	IgG	7 ± 2	17 ± 4

*Significant difference between the unlabeled and IRDye800CW-labeled 8709 Fab (p < 0.0001). ns = no saturation.