Figure 8.

Histological evaluation of new bone formation in the critical-size bone defect model of OVX rats after 12 weeks. (A) Masson's trichrome staining of decalcified bone including full-image and magnified views from different sites in the defect area. FT, fibrous tissue; IB, immature bone; MB, mature bone; and RB, resident BCP. Scale bars, 1 mm, 500 μm; magnification 50×, 200×. (B) Representative full image of undecalcified bone; the section was stained with McNeal's tetrachrome, basic fuchsine, and toluidine blue O. Pink staining represents bone tissue and blue staining represents remaining BCP (original magnification 10×; scale bar, 1 mm). (C) Histomorphometric measurements of the volume density of total newly formed bone (pink) and remaining BCP scaffold (blue) at 12 weeks after implantation according to McNeal's staining. Values are shown as the mean ± S.D. ** P < 0.01, * P < 0.05, indicating significant differences between groups. (D) Representative images showing immunohistochemical staining of CaSR, OCN, PCNA, and ICAM-1 expression in the defect area (original magnification, 100×; scale bar, 100 μm). (E) Quantification data for CaSR, OCN, PCNA, and ICAM-1 expression based on D. Data are presented as the mean ± S.D. Significant differences among groups are indicated as ** P < 0.01, * P < 0.05, n = 5.