FIG 5.
Rates of substrate hydrolysis by HOV Pro, HOV Pro R112A, and NV Pro in the presence of the Syc-10 and Syc-59 aldehyde inhibitors. Substrate hydrolysis was measured by FRET, as described above, in the presence of increasing concentrations of the Syc-10 and Syc-59 inhibitor. The Ki values were determined by fitting the initial velocities to the Morrison tight-binding equation using GraphPad Prism 7. All experiments were carried out in triplicate. The standard errors of Ki were generated by the GraphPad software.