FIG 8.

Evaluation of NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC) effector function in an in vitro killing assay. Purified IgG derived from pooled sera from BALB/c mice immunized with either adjuvanted FILORAB3 (purple) or adjuvanted BNSP333 (blue) was incubated with 3T3-MARV cells with or without addition of an antibody cocktail to block the FcγRIII on the surface of NK cells. NK effector cells were subsequently added at an effector cell-to-target cell ratio of 5:1. Killing was measured by flow cytometry (reported as percent cytotoxicity). As negative controls, MARV-infected 3T3 cells were incubated with purified IgG alone in the absence of NK effector cells (black and brown bars) or with NK effector cells alone and no antibodies (orange bars). Statistical significance was performed using 2-way analysis of variance and post hoc analysis with Tukey’s multiple-comparison test. **, P < 0.01; ****, P < 0.0001; n.s., not significant. Data are representative of average values from triplicate values across 3 independent experiments.