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The alanine-rich domain of RBM24 interacts with the reverse transcriptase domain of HBV polymerase. (A) Schematic illustrations of truncated Pol constructs and truncated RBM24 constructs. (B to D) HEK293T cells were cotransfected with the indicated plasmids and lysed at 48 hpt, followed by a co-IP assay using an anti-Flag antibody. The precipitates were analyzed by Western blotting using anti-Flag and anti-HA antibodies. Actin served as a loading control.
