FIG 5.

RBM24 mediates Pol-ε interaction via the RT domain. HEK293T cells were transfected with the indicated siRNAs or plasmids. (A) The level of RBM24 was detected by Western blotting. (B) Cell lysates were subjected to a biotin-ε pulldown assay. The input or pulled-down proteins were detected by Western blotting. Actin served as a loading control. (C) Cell lysates were subjected to co-IP with an anti-Flag antibody. The precipitates were detected by Western blotting. (D) Cell lysates were subjected to a biotin-ε pulldown assay. The input or pulled-down proteins were detected by Western blotting. Actin served as a loading control.