FIG 6.
AGO4-mediated RdDM plays a role in defense against CLCuMuV infection. (A) Viral symptoms in NbAGO4-silenced plants infected with CLCuMuV at 18 dpi. (B) Southern blot detection showing viral DNA accumulation in NbAGO4-silenced and control plants. A partial CLCuMuV V1 DNA was used as a template to make a 32P-labeled probe. Total DNA was stained with ethidium bromide as a loading control. Single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) corresponding to CLCuMuV are indicated by arrows. (C) Detection of cytosine methylation status of the CLCuMuV 5′ intergenic region (IR) in NbAGO4-silenced or control plants. The histogram represents the proportions of methylated cytosine in different sequence groups. The asterisk indicates significant differences (*, P < 0.05, Student’s t test). The bars represent means ± SD. Data were obtained from three independent experiments.