FIG 5.

Immunodetection of viral proteins in a time course of equivalent amounts of N. benthamiana protoplasts inoculated with Sf-RV (A), Turnip crinkle virus (TCV) (B), or Potato yellow dwarf virus strain (SYDV) (C). Protoplasts were sampled at 12, 16, and 24 h after inoculation and probed via Western immunoblotting with polyclonal antibodies raised against the Sf-RV nucleocapsid (Sf-RV:N), TCV coat protein (TCV:CP), or disrupted SYDV virions, which detect a ladder of proteins corresponding to G, N, M, and P (SYDV). Purified virus (V) and uninoculated protoplasts (C) served as controls.