FIG 3.

LANA and its deletion mutants maintain the ability to bind KSHV TR DNA, as assessed by EMSA. RRL (lane 1) or similar amounts (as assessed by Western blotting) of in vitro-translated LANA (lane 3) or LANA mutants (lanes 4 to 11) were each incubated with radiolabeled TR DNA probe. The lane 2 incubation included anti-T7 antibody, which detects the amino-terminal LANA T7 epitope. After incubation, complexes were resolved on a nondenaturing polyacrylamide gel. Brightness and contrast were uniformly adjusted using Adobe Photoshop. The results are representative of 3 independent experiments. The data are from two gels (separated by a space between lanes 8 and 9) run simultaneously. The thin lines between lanes 2 and 3 and lanes 9 and 10 indicate where lanes from the same gel were not included. The lines on the left indicate LANA or LANA mutant complexes. ▶, *, and # indicate supershifted LANA complexes in lane 2. O, gel origin.