FIG 6.

LANA mutants with deletions including amino acids 194 to 273 are deficient for drug-resistant outgrowth after TR DNA transfection. Limiting-dilution outgrowth assays were performed after p8TR (A and B) or p8TR-P (C and D) transfection into BJAB cells, BJAB cells stably expressing LANA, or BJAB cells stably expressing LANA deletion mutants. Forty-eight hours posttransfection, cells were seeded at an average of 1, 10, 100, or 1,000 cells per well into 96-well microtiter plates and selected for p8TR using G418 (A and B) or puromycin (C and D). Drug-resistant outgrowth was recorded 21 days after cell plating. Averages of the results of three independent experiments are shown in graph form (A and C), or numerical values of the averages are shown (B and D). The error bars indicate standard deviations.