FIG 2.

ICP0 promoter activation by GR and KLF family members. (A) Schematic of full-length ICP0 promoter (FL ICP0) and deletion constructs used for this study. Locations of certain transcription factor binding sites in the FL ICP0 promoter are indicated below the ICP0 constructs. Neuro-2A (B, D, and F) or Vero (C, E, and G) cells were cultured in 2% stripped FBS after transfection with the full-length ICP0 promoter construct. Shown is luciferase activity at 40 h after cells were cotransfected with the FL ICP0 promoter (0.5 μg DNA), vector expressing the mouse GR (1.0 μg of DNA), and/or vector expressing human KLF15 (B and C), KLF4 (D and E), or KLF6 (F and G) (0.5 μg of DNA). Empty vector plasmid was added to certain samples to equalize the total amount of DNA in each transfection. Twenty-four hours following transfection, certain cultures were treated with DEX for 14 h (10 μM). The results are the means from 3 independent experiments. Statistical analysis was performed as described in Materials and Methods. *, P < 0.05; **, P < 0.005; ****, P < 0.0001. ns, not significant.