FIG 3.

Localization of ICP0 promoter sequences that are transactivated by the GR and KLF15. Neuro-2A (A) and Vero (B) cells were cultured in 2% stripped FBS after transfection with the FL ICP0 promoter or designated ICP0 deletion mutant fused to the firefly luciferase gene. Luciferase activity was measured 40 h after cells were cotransfected with the designated ICP0 construct (0.5 μg of DNA), vector expressing the mouse GR (1.0 μg of DNA), and/or vector expressing human KLF15 (0.5 μg of DNA) as indicated. Empty vector plasmid was added to certain samples to maintain the same amount of DNA in each transfection. Twenty-four hours following transfection, designated cultures were treated with DEX for 14 h (10 μM). The results are the means from 3 independent experiments. Statistical analysis was performed as described in Materials and Methods. Unpaired t test was performed between FL ICP0 promoter cotransfected with GR+DEX+KLF15 and indicated samples. *, P < 0.05; **, P < 0.005. N.S., not significant.