FIG 5.

Association of GR and KLF15 with the ICP0 promoter construct. Neuro-2A (A and B) and Vero (C and D) cells were cultured in 2% stripped FBS following transfection with the FL ICP0 promoter and either mouse GR, human KLF15, or empty vector and treated with DEX 24 h later. Cells were cross-linked with 16% paraformaldehyde 48 h following transfection and harvested for ChIP studies. ChIP was performed, as described in Materials and Methods, using the GR antibody, KLF15 antibody, or a control isotype IgG. PCR was performed using the −635 primers, and the DNA was run on a 1% agarose gel to separate DNA fragments and stained with ethidium bromide. Bands were quantified using Image Lab software and graphed as percent input. These results are the means from 3 independent experiments. Samples designated by an asterisk(s) show statistically significant differences from isotype control IgG by the t test (*, P < 0.05; **, P < 0.005).