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. 2019 Mar 5;93(6):e02222-18. doi: 10.1128/JVI.02222-18

FIG 2.

FIG 2

Interaction between PCV2 and heparin-conjugated chromatography sorbent. (A) Binding kinetics between PCV2 VLPs and the chromatography sorbent with 370 nM (black) and 92 nM (red) concentrations of VLP. (B) Elution of PCV2 from the chromatography sorbent in the presence of increasing concentration of heparin. (C) Competition assays testing the ability of various GAGs and polysaccharides to displace PCV2 from the chromatography sorbent. Red, dp6 heparin (6-hexose); blue, dp10 heparin (10-hexose); dark gray, dp20 heparin (20-hexose); black, dp36 heparin (36-hexose); magenta, 8-kDa dextran sulfate; brown, 6-kDa dextran sulfate; light gray, 41-kDa chondroitin sulfate B; green, hyaluronic acid. Error bars represent the standard deviation for three measurements. Images were made with Origin 2016 software (OriginLab).