TABLE 2.

Bacterial strains used in this study^a

Strain identifier	LTEE population	Generation
REL606	Ancestral strain	0
REL1158A	Ara+1	2,000
REL1159A	Ara+2	2,000
REL1160A	Ara+3	2,000
REL1161A	Ara+4	2,000
REL1162A	Ara+5	2,000
REL1163A	Ara+6	2,000
REL1164A	Ara–1	2,000
REL1165A	Ara–2	2,000
REL1166A	Ara–3	2,000
REL1167A	Ara–4	2,000
REL1168A	Ara–5	2,000
REL1169A	Ara–6	2,000
REL11392	Ara+1	50,000
REL11342	Ara+2	50,000
REL11345b	Ara+3	50,000
REL11348	Ara+4	50,000
REL11367	Ara+5	50,000
REL11370b	Ara+6	50,000
REL11330b	Ara–1	50,000
REL11335b	Ara–2	50,000
REL11364b	Ara–3	50,000
REL11336b	Ara–4	50,000
REL11339	Ara–5	50,000
REL11389	Ara–6	50,000

^aAll strains derive from E. coli B REL606 or REL607, an Ara+ mutant of REL606 (17, 19).

^bThese clones are from populations that were hypermutable for much of the time during the LTEE.