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. 2019 Mar 5;10(2):e02567-18. doi: 10.1128/mBio.02567-18

FIG 3.

FIG 3

The ATPase Vps4 is reduced in the TG after HSV-1 corneal infection. (A) ESCRT-III proteins Chmp4B and Chmp2B were analyzed by WB using TG protein extracts from corneally infected LC3-GFP+/− (1 × 10e6 PFU/eye, HSV-1 strain 17) mice and analyzed at the indicated times. Each line corresponds to a single TG. Graph shows quantification of WBs normalized to β-actin; n = 6 TG from 6 mice, performed in two independent experiments. **, P < 0.01. (B) Vps4 was analyzed by WB using TG protein extracts from infected LC3-GFP+/− mice (1 × 10e6 PFU/eye, HSV-1 strain 17) at the indicated times. Each line corresponds to a single TG. Graph shows quantification of WBs normalized to β-actin; n = 9 TG from 9 different mice, performed in three independent experiments. **, P < 0.01; ***, P < 0.001. Vps4 antibody recognizes both Vps4A and Vps4B.