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. 2019 Mar 5;10(2):e02567-18. doi: 10.1128/mBio.02567-18

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Copyright © 2019 Cabrera et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — LC3 clusters are associated with ubiquitin accumulations. (A) Representative images from immunofluorescence microscopy of cultured TG neurons from LC3-GFP^+/− mice that were either untreated (upper row) or treated (lower row) with IFN β+γ for 4 days. LC3-GFP is in green; ubiquitin is shown in magenta. The experiment shown is representative of three independent experiments; >1,000 neurons were analyzed. (B) Representative stack from high-resolution (Airyscan, Zeiss) confocal microscopy of TG neurons from LC3-GFP^+/− mice in culture treated with IFN β+γ for 4 days (upper row). Lower row shows the magnification and deconvolution of the above LC3 cluster and ubiquitin accumulation. LC3-GFP is in green; ubiquitin is shown in magenta.