TABLE 2.
Ferredoxin interactomes identified in T. kodakarensis
| TK gene no. | Protein function | S0
condition |
No. of unique peptides |
% of coverage |
No. of normalized total spectra |
|---|---|---|---|---|---|
| Proteins copurified with TK1694 (Fd-1) |
|||||
| TK1694 | Ferredoxin 1 | −S0/+S0 | 2 | 64 | 2,569 |
| TK1980 | 2-Oxoisovalerate:ferredoxin oxidoreductase, α subunit | −S0/+S0 | 24 | 82 | 1,366 |
| TK1981 | 2-Oxoisovalerate:ferredoxin oxidoreductase, β subunit | −S0/+S0 | 17 | 76 | 1,314 |
| TK1978 | 2-Oxoisovalerate ferredoxin oxidoreductase, γ subunit | −S0/+S0 | 16 | 87 | 902 |
| TK1325 | Ferredoxin:NADH oxidoreductase, α subunit | −S0/+S0 | 25 | 66 | 319 |
| TK1979 | 2-Oxoisovalerate ferredoxin oxidoreductase, δ subunit | −S0/+S0 | 8 | 88 | 290 |
| TK0650 | Rubrerythrin-related protein | −S0/+S0 | 15 | 67 | 157 |
| TK1326 | Ferredoxin:NADH oxidoreductase, β subunit | −S0/+S0 | 17 | 65 | 135 |
| TK1684 | Ferredoxin:NADP oxidoreductase, α subunit | −S0/+S0 | 23 | 65 | 125 |
| TK1983 | Pyruvate:ferredoxin oxidoreductase, α subunit | −S0/+S0 | 8 | 30 | 48 |
| TK1685 | Ferredoxin:NADH oxidoreductase, β subunit | −S0/+S0 | 8 | 39 | 40 |
| TK1984 | Pyruvate:ferredoxin oxidoreductase, β subunit | −S0/+S0 | 6 | 22 | 31 |
| TK0537 | Peroxiredoxin | +S0 | 2 | 10 | 19 |
| TK1982 | Pyruvate:ferredoxin oxidoreductase, δ subunit | −S0/+S0 | 2 | 33 | 13 |
| TK0136 | Indolepyruvate:ferredoxin oxidoreductase, α subunit | −S0/+S0 | 6 | 11 | 13 |
| TK0135 | Indolepyruvate:ferredoxin oxidoreductase, β subunit | −S0/+S0 | 1 | 4 | 2 |
| Proteins copurified with TK1087 (Fd-2) |
|||||
| TK1087 | Ferredoxin 2 | −S0/+S0 | 3 | 87 | 28 |
| TK2076 | Formate dehydrogenase, α subunit | −S0/+S0 | 31 | 54 | 887 |
| TK1088 | Geranylgeranyl hydrogenase | −S0/+S0 | 22 | 61 | 312 |
| TK2077 | Formate dehydrogenase, 4Fe-4S cluster-binding protein | −S0/+S0 | 10 | 67 | 185 |
| TK2303 | Thermosome, β subunit | −S0/+S0 | 21 | 54 | 182 |
| TK0799 | Type II DNA topoisomerase VI, subunit B | −S0/+S0 | 21 | 41 | 149 |
| TK1325 | Ferredoxin:NADH oxidoreductase, α subunit | +S0 | 16 | 51 | 133 |
| TK1326 | Ferredoxin:NADH oxidoreductase, β subunit | +S0 | 13 | 65 | 130 |
| TK0798 | Type II DNA topoisomerase VI, subunit A | −S0/+S0 | 12 | 30 | 119 |
| TK2078 | 4Fe-4S cluster-binding protein associated with formate dehydrogenase | −S0/+S0 | 9 | 51 | 99 |
| TK0893 | Pyruvate formate-lyase activating enzyme-related protein | −S0/+S0 | 9 | 34 | 77 |
| TK0678 | Thermosome, α subunit | −S0/+S0 | 13 | 45 | 52 |
| TK2075 | 4Fe-4S cluster-binding protein associated with formate dehydrogenase | −S0/+S0 | 4 | 38 | 44 |
| TK1983 | Pyruvate:ferredoxin oxidoreductase, α subunit | −S0/+S0 | 2 | 7 | 23 |
| TK1984 | Pyruvate:ferredoxin oxidoreductase, β subunit | −S0/+S0 | 3 | 15 | 21 |
| TK1130 | 2-Oxoacid:ferredoxin oxidoreductases, α subunit | −S0/+S0 | 6 | 20 | 21 |
| TK1056 | Rubrerythrin-related protein | −S0/+S0 | 2 | 22 | 17 |
| TK1123 | Oxoacid:ferredoxin oxidoreductases, γ subunit | −S0/+S0 | 3 | 23 | 15 |
| TK0136 | Indolepyruvate:ferredoxin oxidoreductase, α subunit | −S0/+S0 | 3 | 7 | 10 |
| TK2072 | Cytosolic NiFe-hydrogenase, β subunit | −S0 | 2 | 7 | 7 |
| TK2091 | Membrane-bound hydrogenase, MbhL | −S0 | 3 | 10 | 6 |
| TK1684 | Ferredoxin:NADP oxidoreductase, α subunit | −S0 | 4 | 9 | 6 |
| TK1055 | Peroxiredoxin | −S0/+S0 | 2 | 16 | 5 |
| TK2021 | ATPase near Fe-transport operon | −S0/+S0 | 3 | 17 | 5 |
| TK1685 | Ferredoxin:NADH oxidoreductase, β subunit | −S0 | 2 | 10 | 4 |
| TK1129 | 2-Oxoacid:ferredoxin oxidoreductases, β subunit | +S0 | 3 | 17 | 4 |
| Proteins copurified with TK2012 (Fd-3) |
|||||
| TK2012 | Ferredoxin 3 | −S0 | 21 | 77 | 77 |
| TK2076 | Formate dehydrogenase, α subunit | −S0 | 38 | 55 | 125 |
| TK2091 | Membrane-bound hydrogenase subunit, MbhL | −S0 | 5 | 10 | 27 |
| TK1983 | Pyruvate:ferredoxin oxidoreductase, α subunit | −S0 | 2 | 7 | 23 |
| TK1085 | Protein disulfide oxidoreductase | −S0 | 4 | 19 | 21 |
| TK1596 | V-type ATP synthase, subunit H | −S0 | 6 | 59 | 18 |
| TK2090 | Membrane-bound hydrogenase subunit, MbhK | −S0 | 6 | 47 | 15 |
| TK1982 | Pyruvate:ferredoxin oxidoreductase, δ subunit | −S0 | 2 | 33 | 13 |
| TK1978 | 2-Oxoisovalerate ferredoxin oxidoreductase, γ subunit | −S0 | 4 | 25 | 12 |
| TK1979 | 2-Oxoisovalerate ferredoxin oxidoreductase, δ subunit | −S0 | 3 | 47 | 11 |
| TK2089 | Membrane-bound hydrogenase subunit, MbhJ | −S0 | 4 | 24 | 9 |
| TK0798 | Type II DNA topoisomerase VI, subunit A | −S0 | 8 | 23 | 9 |
| TK0537 | Peroxiredoxin | −S0 | 3 | 19 | 8 |
| TK1602 | V-type ATP synthase, subunit A | −S0 | 4 | 9 | 7 |
| TK2303 | Thermosome, β subunit | −S0 | 5 | 11 | 6 |
| TK2075 | 4Fe-4S cluster-binding protein associated with formate dehydrogenase | −S0 | 4 | 32 | 6 |
| TK1603 | V-type ATP synthase, subunit B | −S0 | 2 | 14 | 5 |
| TK0525 | Superoxide reductase | −S0 | 2 | 20 | 4 |
| TK1604 | V-type ATP synthase subunit D | −S0 | 2 | 9 | 3 |
| TK2077 | Formate dehydrogenase, 4Fe-4S cluster-binding protein | −S0 | 3 | 23 | 3 |
| TK1984 | Pyruvate:ferredoxin oxidoreductase, β subunit | −S0 | 2 | 12 | 2 |
| TK0799 | Type II DNA topoisomerase VI, subunit B | −S0 | 2 | 5 | 2 |
| TK1981 | 2-Oxoisovalerate:ferredoxin oxidoreductase, β subunit | −S0 | 1 | 5 | 1 |
| TK2088 | Membrane-bound hydrogenase subunit, MbhI | −S0 | 1 | 8 | 1 |