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. 2019 Mar 5;10(2):e02807-18. doi: 10.1128/mBio.02807-18

TABLE 2.

Ferredoxin interactomes identified in T. kodakarensis

TK gene no. Protein function S0
condition
No. of
unique
peptides
% of
coverage
No. of
normalized
total spectra
Proteins copurified
with TK1694 (Fd-1)
    TK1694 Ferredoxin 1 −S0/+S0 2 64 2,569
    TK1980 2-Oxoisovalerate:ferredoxin oxidoreductase, α subunit −S0/+S0 24 82 1,366
    TK1981 2-Oxoisovalerate:ferredoxin oxidoreductase, β subunit −S0/+S0 17 76 1,314
    TK1978 2-Oxoisovalerate ferredoxin oxidoreductase, γ subunit −S0/+S0 16 87 902
    TK1325 Ferredoxin:NADH oxidoreductase, α subunit −S0/+S0 25 66 319
    TK1979 2-Oxoisovalerate ferredoxin oxidoreductase, δ subunit −S0/+S0 8 88 290
    TK0650 Rubrerythrin-related protein −S0/+S0 15 67 157
    TK1326 Ferredoxin:NADH oxidoreductase, β subunit −S0/+S0 17 65 135
    TK1684 Ferredoxin:NADP oxidoreductase, α subunit −S0/+S0 23 65 125
    TK1983 Pyruvate:ferredoxin oxidoreductase, α subunit −S0/+S0 8 30 48
    TK1685 Ferredoxin:NADH oxidoreductase, β subunit −S0/+S0 8 39 40
    TK1984 Pyruvate:ferredoxin oxidoreductase, β subunit −S0/+S0 6 22 31
    TK0537 Peroxiredoxin +S0 2 10 19
    TK1982 Pyruvate:ferredoxin oxidoreductase, δ subunit −S0/+S0 2 33 13
    TK0136 Indolepyruvate:ferredoxin oxidoreductase, α subunit −S0/+S0 6 11 13
    TK0135 Indolepyruvate:ferredoxin oxidoreductase, β subunit −S0/+S0 1 4 2
Proteins copurified
with TK1087 (Fd-2)
    TK1087 Ferredoxin 2 −S0/+S0 3 87 28
    TK2076 Formate dehydrogenase, α subunit −S0/+S0 31 54 887
    TK1088 Geranylgeranyl hydrogenase −S0/+S0 22 61 312
    TK2077 Formate dehydrogenase, 4Fe-4S cluster-binding protein −S0/+S0 10 67 185
    TK2303 Thermosome, β subunit −S0/+S0 21 54 182
    TK0799 Type II DNA topoisomerase VI, subunit B −S0/+S0 21 41 149
    TK1325 Ferredoxin:NADH oxidoreductase, α subunit +S0 16 51 133
    TK1326 Ferredoxin:NADH oxidoreductase, β subunit +S0 13 65 130
    TK0798 Type II DNA topoisomerase VI, subunit A −S0/+S0 12 30 119
    TK2078 4Fe-4S cluster-binding protein associated with formate dehydrogenase −S0/+S0 9 51 99
    TK0893 Pyruvate formate-lyase activating enzyme-related protein −S0/+S0 9 34 77
    TK0678 Thermosome, α subunit −S0/+S0 13 45 52
    TK2075 4Fe-4S cluster-binding protein associated with formate dehydrogenase −S0/+S0 4 38 44
    TK1983 Pyruvate:ferredoxin oxidoreductase, α subunit −S0/+S0 2 7 23
    TK1984 Pyruvate:ferredoxin oxidoreductase, β subunit −S0/+S0 3 15 21
    TK1130 2-Oxoacid:ferredoxin oxidoreductases, α subunit −S0/+S0 6 20 21
    TK1056 Rubrerythrin-related protein −S0/+S0 2 22 17
    TK1123 Oxoacid:ferredoxin oxidoreductases, γ subunit −S0/+S0 3 23 15
    TK0136 Indolepyruvate:ferredoxin oxidoreductase, α subunit −S0/+S0 3 7 10
    TK2072 Cytosolic NiFe-hydrogenase, β subunit −S0 2 7 7
    TK2091 Membrane-bound hydrogenase, MbhL −S0 3 10 6
    TK1684 Ferredoxin:NADP oxidoreductase, α subunit −S0 4 9 6
    TK1055 Peroxiredoxin −S0/+S0 2 16 5
    TK2021 ATPase near Fe-transport operon −S0/+S0 3 17 5
    TK1685 Ferredoxin:NADH oxidoreductase, β subunit −S0 2 10 4
    TK1129 2-Oxoacid:ferredoxin oxidoreductases, β subunit +S0 3 17 4
Proteins copurified
with TK2012 (Fd-3)
    TK2012 Ferredoxin 3 −S0 21 77 77
    TK2076 Formate dehydrogenase, α subunit −S0 38 55 125
    TK2091 Membrane-bound hydrogenase subunit, MbhL −S0 5 10 27
    TK1983 Pyruvate:ferredoxin oxidoreductase, α subunit −S0 2 7 23
    TK1085 Protein disulfide oxidoreductase −S0 4 19 21
    TK1596 V-type ATP synthase, subunit H −S0 6 59 18
    TK2090 Membrane-bound hydrogenase subunit, MbhK −S0 6 47 15
    TK1982 Pyruvate:ferredoxin oxidoreductase, δ subunit −S0 2 33 13
    TK1978 2-Oxoisovalerate ferredoxin oxidoreductase, γ subunit −S0 4 25 12
    TK1979 2-Oxoisovalerate ferredoxin oxidoreductase, δ subunit −S0 3 47 11
    TK2089 Membrane-bound hydrogenase subunit, MbhJ −S0 4 24 9
    TK0798 Type II DNA topoisomerase VI, subunit A −S0 8 23 9
    TK0537 Peroxiredoxin −S0 3 19 8
    TK1602 V-type ATP synthase, subunit A −S0 4 9 7
    TK2303 Thermosome, β subunit −S0 5 11 6
    TK2075 4Fe-4S cluster-binding protein associated with formate dehydrogenase −S0 4 32 6
    TK1603 V-type ATP synthase, subunit B −S0 2 14 5
    TK0525 Superoxide reductase −S0 2 20 4
    TK1604 V-type ATP synthase subunit D −S0 2 9 3
    TK2077 Formate dehydrogenase, 4Fe-4S cluster-binding protein −S0 3 23 3
    TK1984 Pyruvate:ferredoxin oxidoreductase, β subunit −S0 2 12 2
    TK0799 Type II DNA topoisomerase VI, subunit B −S0 2 5 2
    TK1981 2-Oxoisovalerate:ferredoxin oxidoreductase, β subunit −S0 1 5 1
    TK2088 Membrane-bound hydrogenase subunit, MbhI −S0 1 8 1