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Representative images of CTC subtypes: CD133 positive (top row) and CD133 negative (bottom row) subtypes. All 'CTC subtypes' were isolated in EpCAM-functionalized devices and confirmed using the FDA-approved definition of a CTC (DAPI+CK+CD45-). Expression of CD133 (red), CK (green), CD45 (yellow), and DAPI (blue) was determined using fluorescent staining and imaging. Scale bar represents 20 µm.
