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. 2019 Jan 31;9(5):1232–1246. doi: 10.7150/thno.25735

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Evaluation of the effect of bungarotoxin in NMJ function. (A-B) Contractility traces and light stimulation protocol of a representative tissue before (A) and after (B) 20 min treatment with 5 mg/ml of the neurotoxin α-bungarotoxin (BTX). (C) Quantification of tissue responsiveness to light (score), represented as the corrected fraction of effective light pulses before and after treatment with BTX (n = 6; one-way ANOVA F=9·10^-8). (D) Reduction of the number of spontaneous contractions after treatment with BTX (n = 6; one-way ANOVA F=0.03). BTX was added at day 18 after the beginning of the coculture. Error bars represent the standard error of the mean (SEM).