Figure 2.

Binding affinity and quality control of PSPS. (A) and (B). Detection and statistical analysis of the binding affinity of PSMAb, PSP, and PSPS by ELISA. (C). Specific siRNA payload estimation of PSP. Different molar ratios of PSP complexes to FAM-siRNA were incubated for 2h and gel shift assays were performed. (D). Specific FAM-siRNA payload of PSP was analyzed by the fluorescent intensity of FAM-siRNA bound to PSP captured on protein A/G agarose beads. (E). Protective effect of PSP for FAM-siRNA (PSP/FAM-siRNA:1/5) against enzymatic digestion. (F). Fluorescence intensity assay ns: not significant vs control group. Data are shown as the mean ± S.E. ns: not significant. Representative results of 3 independent experiments are shown.