Figure 4.

Specific enrichment of PSP and PSPS in PSMA+ CRPC xenografts in vivo. (A) Dynamic whole-body and (B) major organs distribution of ICG-labeled PSP in vivo. ICG-labeled PSP was injected into PC-3-PSMA+ xenografted nude mice. The whole-body distribution of ICG-labeled PSP was monitored at indicated time points, and the resected major organs distribution was monitored at 96 h after ICG-labeled PSP injection by fluorescence imaging excited at 745 nm. BLI was acquired to identify the CRPC tumor tissues. (C) Dynamic whole-body and (D) major organs distribution of PSPS in vivo. PSPS was injected into PC-3-PSMA+ xenograft nude mice. The whole-body distribution of PSPS was monitored at indicated time points, and the resected major organ distribution was monitored at 96 h after PSPS injection by fluorescence imaging excited at 488 nm. BLI was acquired to identify the CRPC tumor tissues. IgG-P-FAM-siRNA and FAM-siRNA were used as negative controls. Tumors and major organs were resected 96h after PSPS injection. Fluorescence images of (E) tumor and (F) major organs cryosections were taken by laser scanning confocal microscopy. IgG-P-FAM-siRNA and FAM-siRNA were used as negative controls. Scale bars = 50 μm. Data are shown as the mean ± S.E. Representative results of 3 independent experiments are shown.