. 2019 Feb 28;5(2):e01270. doi: 10.1016/j.heliyon.2019.e01270

Table 1.

List of primers, probes and positive controls used for the fluidigm array. Most primers and probes as well as the positive controls were already used and described in a previous study [14]. Length of the PCR product in base pairs (bp).

Pathogens	Target	Primers	Sequence	Length	Positive control
Borrelia burgdorferi	rpoB	Bo_bu_rpoB_F Bo_bu_rpoB_R Bo_bu_rpoB_P	GCTTACTCACAAAAGGCGTCTT GCACATCTCTTACTTCAAATCCT AATGCTCTTGGACCAGGAGGACTTTCA	83 bp	Culture of B31
Borrelia garinii	rpoB	Bo_ga_rpoB_F Bo_ga_rpoB_R Bo_ga_rpoB_P	TGGCCGAACTTACCCACAAAA ACATCTCTTACTTCAAATCCTGC TCTATCTCTTGAAAGTCCCCCTGGTCC	88 bp	Culture of NE11
Borrelia afzelii	flaB	Bo_af_fla_F Bo_af_fla_R Bo_af_fla_P	GGAGCAAATCAAGATGAAGCAAT TGAGCACCCTCTTGAACAGG TGCAGCCTGAGCAGCTTGAGCTCC	116 bp	Culture of VS641
Borrelia valaisiana	ospA	Bo_va_ospA_F Bo_va_ospA_R Bo_va_ospA_P	ACTCACAAATGACAGATGCTGAA GCTTGCTTAAAGTAACAGTACCT TCCGCCTACAAGATTTCCTGGAAGCTT	135 bp	Culture of VS116
Borrelia miyamotoi	glpQ	B_miy_glpQ_F B_miy_glpQ_R B_miy_glpQ_P	CACGACCCAGAAATTGACACA GTGTGAAGTCAGTGGCGTAAT TCGTCCGTTTTCTCTAGCTCGATTGGG	94 bp	Plasmid^a
Borrelia spielmanii	fla	Bo_sp_fla_F Bo_sp_fla_R Bo_sp_fla_P	ATCTATTTTCTGGTGAGGGAGC TCCTTCTTGTTGAGCACCTTC TTGAACAGGCGCAGTCTGAGCAGCTT	71 bp	Plasmid
Borrelia lusitaniae	rpoB	Bo_lu_rpoB_F Bo_lu_rpoB_R Bo_lu_rpoB_P	CGAACTTACTCATAAAAGGCGTC TGGACGTCTCTTACTTCAAATCC TTAATGCTCTCGGGCCTGGGGGACT	87 bp	Culture of Poti-B1
Borrelia bissettii	rpoB	Bo_bi_rpoB_F Bo_bi_rpoB_R Bo_bi_rpoB_P	GCAACCAGTCAGCTTTCACAG CAAATCCTGCCCTATCCCTTG AAAGTCCTCCCGGCCCAAGAGCATTAA	118 bp	Plasmid^a
Borrelia spp.	23SrRNA	Bo_sl_23S_F Bo_sl_23S_R Bo_sl_23S_P	GAGTCTTAAAAGGGCGATTTAGT CTTCAGCCTGGCCATAAATAG AGATGTGGTAGACCCGAAGCCGAGT	73 bp	Culture of B31
Anaplasma marginale	msp1b	An_ma_msp1_F An_ma_msp1_R An_ma_msp1_P	CAGGCTTCAAGCGTACAGTG GATATCTGTGCCTGGCCTTC ATGAAAGCCTGGAGATGTTAGACCGAG	85 bp	Experimentally infected cow
Anaplasma platys	groEL	An_pl_groEL_F An_pl_groEL_R An_pl_groEL_P	TTCTGCCGATCCTTGAAAACG CTTCTCCTTCTACATCCTCAG TTGCTAGATCCGGCAGGCCTCTGC	75 bp	Dog blood
Anaplasma ovis	msp4	An_ov_msp4_F An_ov_msp4_R An_ov_msp4_P	TCATTCGACATGCGTGAGTCA TTTGCTGGCGCACTCACATC AGCAGAGAGACCTCGTATGTTAGAGGC	92 bp	Plasmid^a
Anaplasma bovis	groEL	An_bov_groEL_F An_bov_groEL_R An_bov_groEL_P	GGGAGATAGTACACATCCTTG CTGATAGCTACAGTTAAGCCC AGGTGCTGTTGGATGTACTGCTGGACC	73 bp	Plasmid^a
Anaplasma centrale	groEL	An_ce_groEL_F An_ce_groEL_R An_ce_groEL_P	AGCTGCCCTGCTATACACG GATGTTGATGCCCAATTGCTC CTTGCATCTCTAGACGAGGTAAAGGGG	79 bp	Plasmid^a
Anaplasma phagocytophilum	msp2	An_ph_msp2_F An_ph_msp2_R An_ph_msp2_P	GCTATGGAAGGCAGTGTTGG GTCTTGAAGCGCTCGTAACC AATCTCAAGCTCAACCCTGGCACCAC	77 bp	Culture
Ehrlichia ruminantium	dsb	Eh_ru_dsb_F Eh_ru_dsb_R Eh_ru_dsb_P	CTCAGAGGGTAATAGATTTACTC GTATGCAATATCTTCAAGCTCAG ACTACAGGCCAAGCACAAGCAGAAAGA	107 bp	Culture of Gardel
Ehrlichia canis	dsb	Eh_ca_dsb_F Eh_ca_dsb_R Eh_ca_dsb_P	AATACTTGGTGAGTCTTCACTCA GTTGCTTGTAATGTAGTGCTGC AAGTTGCCCAAGCAGCACTAGCTGTAC	110 bp	Plasmid^a
Ehrlichia chaffeensis	dsb	Eh_ch_dsb_F Eh_ch_dsb_R Eh_ch_dsb_P	TATTGCTAATTACCCTCAAAAAGTC GAGCTATCCTCAAGTTCAGATTT ATTGACCTCCTAACTAGAGGGCAAGCA	117 bp	Amblyomma americanum
Neoehrlichia mikurensis	groEL	Nm_groEL_F Nm_groEL_R Nm_groEL_P	AGAGACATCATTCGCATTTTGGA TTCCGGTGTACCATAAGGCTT AGATGCTGTTGGATGTACTGCTGGACC	96 bp	Ixodes ricinus
Rickettsia conorii	23S-5S ITS	Ri_co_ITS_F Ri_co_ITS_R Ri_co_ITS_P	CTCACAAAGTTATCAGGTTAAATAG CGATACTCAGCAAAATAATTCTCG CTGGATATCGTGGCAGGGCTACAGTAT	118 bp	Culture
Rickettsia slovaca	23S-5S ITS	Ri_sl_ITS_F Ri_sl_ITS_R Ri_sl_ITS_P	GTATCTACTCACAAAGTTATCAGG CTTAACTTTTACTACAATACTCAGC TAATTTTCGCTGGATATCGTGGCAGGG	138 bp	Culture
Rickettsia massiliae	23S-5S ITS	Ri_ma_ITS_F Ri_ma_ITS_R Ri_ma_ITS_P	GTTATTGCATCACTAATGTTATACTG GTTAATGTTGTTGCACGACTCAA TAGCCCCGCCACGATATCTAGCAAAAA	128 bp	Culture
Rickettsia helvetica	23S-5S ITS	Ri_he_ITS_F Ri_he_ITS_R Ri_he_ITS_P	AGAACCGTAGCGTACACTTAG GAAAACCCTACTTCTAGGGGT TACGTGAGGATTTGAGTACCGGATCGA	79 bp	Culture
Rickettsia aeschlimannii	ITS	Rick_aesch_ITS_F Rick_aesch_ITS_R Rick_aesch_ITS_P	CTCACAAAGTTATCAGGTTAAATAG CTTAACTTTTACTACGATACTTAGCA TAATTTTTGCTGGATATCGTGGCGGGG	134 bp	Culture
Spotted fever group	gltA	SFG_gltA_F SFG_gltA_R SFG_gltA_P	CCTTTTGTAGCTCTTCTCATCC GCGATGGTAGGTATCTTAGCAA TGGCTATTATGCTTGCGGCTGTCGGT	145 bp
Bartonella henselae	pap31	Bar_he_pap_F Bar_he_pap_R Bar_he_pap_P	CCGCTGATCGCATTATGCCT AGCGATTTCTGCATCATCTGCT ATGTTGCTGGTGGTGTTTCCTATGCAC	107 bp	Culture of Berlin 1
Bartonella quintana	bqtR	Bar_qu_bqt_F Bar_qu_bqt_R Bar_qu_bqt_P	TCCATCACAAGATCTCCGCG CGTGCCAATGCTCGTAACCA TTTAAGAGAGGAGGTAGAAGAGGCTCC	80 bp	Culture
Francisella tularensis and Francisella-like endosymbionts	tul4	Fr_tu_tul4_F Fr_tu_tul4_R Fr_tu_tul4_P	ACCCACAAGGAAGTGTAAGATTA GTAATTGGGAAGCTTGTATCATG AATGGCAGGCTCCAGAAGGTTCTAAGT	76 bp	Culture of CIP 5612T
	fopA	Fr_tu_fopA_F Fr_tu_fopA_R Fr_tu_fopA_P	GGCAAATCTAGCAGGTCAAGC CAACACTTGCTTGAACATTTCTAG AACAGGTGCTTGGGATGTGGGTGGTG	91 bp	Culture of CIP 5612T
Coxiella burnetii and Coxiella-like	icd	Co_bu_icd_F Co_bu_icd_R Co_bu_icd_P	AGGCCCGTCCGTTATTTTACG CGGAAAATCACCATATTCACCTT TTCAGGCGTTTTGACCGGGCTTGGC	74 bp	Culture
Coxiella burnetii and Coxiella-like	IS1111	Co_bu_IS_F Co_bu_IS_R Co_bu_IS_P	TGGAGGAGCGAACCATTGGT CATACGGTTTGACGTGCTGC ATCGGACGTTTATGGGGATGGGTATCC	86 bp	Culture
Babesia divergens	hsp70	Bab_di_hsp70_F Bab_di_hsp70_R Bab_di_hsp70_P	CTCATTGGTGACGCCGCTA CTCCTCCCGATAAGCCTCTT AGAACCAGGAGGCCCGTAACCCAGA	83 bp	Culture of RFS
Babesia caballi	RAP1	Ba_ca_rap1_F Ba_ca_rap1_R Ba_ca_rap1_P	GTTGTTCGGCTGGGGCATC CAGGCGACTGACGCTGTGT TCTGTCCCGATGTCAAGGGGCAGGT	94 bp	Plasmid^a
Babesia canis (3 subspecies)	RNA 18S	Ba_ca_18S_F Ba_ca_18S_R Ba_ca_18S_P	TGGCCGTTCTTAGTTGGTGG AGAAGCAACCGGAAACTCAAATA ACCGGCACTAGTTAGCAGGTTAAGGTC	104 bp	Dog blood
Babesia canis vogeli	hsp70	Ba_vo_hsp70_F Ba_vo_hsp70_R Ba_vo_hsp70_P	TCACTGTGCCTGCGTACTTC TGATACGCATGACGTTGAGAC AACGACTCCCAGCGCCAGGCCAC	87 bp	Dog blood
Babesia venatorum (EU1)	RNA 18S	Ba_EU_18S_F Ba_EU_18S_R Ba_EU_18S_P	GCGCGCTACACTGATGCATT CAAAAATCAATCCCCGTCACG CATCGAGTTTAATCCTGTCCCGAAAGG	91 bp	Plasmid^a
Babesia microti	CCTeta	Ba_mi_CCT_F Ba_mi_CCT_R Ba_mi_CCT_P	ACAATGGATTTTCCCCAGCAAAA GCGACATTTCGGCAACTTATATA TACTCTGGTGCAATGAGCGTATGGGTA	145 bp	Culture of R1
Babesia bovis	CCTeta	Ba_bo_CCT_F Ba_bo_CCT_R Ba_bo_CCT_P	GCCAAGTAGTGGTAGACTGTA GCTCCGTCATTGGTTATGGTA TAAAGACAACACTGGGTCCGCGTGG	100 bp	Culture of MO7
Babesia bigemina	RNA 18S	Ba_bi_18S_F Ba_bi_18S_R Ba_bi_18S_P	ATTCCGTTAACGAACGAGACC TTCCCCCACGCTTGAAGCA CAGGAGTCCCTCTAAGAAGCAAACGAG	99 bp	Plasmid^a
Babesia major	CCTeta	Ba_ma_CCT_F Ba_ma_CCT_R Ba_ma_CCT_P	CACTGGTGCGCTGATCCAA TCCTCGAAGCATCCACATGTT AACACTGTCAACGGCATAAGCACCGAT	75 bp	Plasmid^a
Babesia ovis	RNA 18S	Ba_ov_18S_F Ba_ov_18S_R Ba_ov_18S_P	TCTGTGATGCCCTTAGATGTC GCTGGTTACCCGCGCCTT TCGGAGCGGGGTCAACTCGATGCAT	92 bp	Plasmid^a
Theileria equi	ema1	Th_eq_ema1_F Th_eq_ema1_R Th_eq_ema1_P	GGCTCCGGCAAGAAGCACA CTTGCCATCGACGACCTTGA CTTCAAGGCTCCAGGCAAGCGCGT	66 bp	Plasmid^a
Theileria annulata	RNA 18S	Th_an_18S_F Th_an_18S_R Th_an_18S_P	GCGGTAATTCCAGCTCCAATA AAACTCCGTCCGAAAAAAGCC ACATGCACAGACCCCAGAGGGACAC	126 bp	Culture of D7
Ixodes ricinus	ITS2	Ix_ri_ITS2_F Ix_ri_ITS2_R Ix_ri_ITS2_P	CGAAACTCGATGGAGACCTG ATCTCCAACGCACCGACGT TTGTGGAAATCCCGTCGCACGTTGAAC	77 bp	Tick
Ixodes persulcatus	ITS2	Ix_pe_ITS2_F Ix_pe_ITS2_R Ix_pe_ITS2_P	TGCGTTGCGTCTTCTCTTGTT TCGATAAAACCAGGTAGGAGGA TTTCGGAGCAAGTACAGAGGGAGCAAA	111 bp	Tick
Dermacentor reticulatus	ITS2	De_re_ITS2_F De_re_ITS2_R De_re_ITS2_P	AACCCTTTTCCGCTCCGTG TTTTGCTAGAGCTCGACGTAC TACGAAGGCAAACAACGCAAACTGCGA	83 bp	Tick
Dermacentor marginatus	ITS2	De_ma_ITS2_F De_ma_ITS2_R De_ma_ITS2_P	GCACGTTGCGTTGTTTGCC CCGCTCCGCGCAAGAATCT TTCGGAGTACGTCGAGCTCTAGCAGA	139 bp	Tick
Escherichia coli	eae	eae-F eae-R eae-P	CATTGATCAGGATTTTTCTGGTGATA CTCATGCGGAAATAGCCGTTA ATAGTCTCGCCAGTATTCGCCACCAATACC	102 bp	Culture of EDL933

Plasmids are recombinant pBluescript IISK+ containing the target gene.