Table 4.
Confirmed presence of tick-borne pathogen DNA in D. reticulatus. Samples which were positive in the microfluidic array were retested by other qPCR or PCR tests (Table 2) to confirm the presence of DNA of a tick-borne pathogen.
| Pathogen | Fluidigm positive | Confirmed (from Table 2) | Countries |
|---|---|---|---|
| Borrelia spp. | 120 | No | |
| B. burgdorferi s.l. | 1 | 1 (qPCR) | Germany |
| B. miyamotoi | 3 | No | |
| Anaplasma marginale | 1 | No | |
| A. phagocytophilum | 5 | No | |
| E. canis | 26 | No | |
| Rickettsia helvetica | 4 | No | |
| SFG Rickettsia(R. raoultii) | 167 | 128 (qPCR), 103 (PCR/Seq) | All countries |
| F. tularensis and FLEs | 1655 | No | |
| Coxiella burnetii and Coxiella-like | 1 | No | |
| Babesia canis | 16 | 16 (qPCR), 10 (PCR/Seq) | Great Britain |
| B. divergens | 3 | No | |
| B. vogeli | 87 | No | |
| Theileria equi | 1 | No |