Fig. 3. Analyses of paravascular area, brain water content, and Tau levels. (A) H&E staining showed that hippocampal blood vessels had smaller inner diameters with increased perivascular space (arrowhead) at 7 days post SAH. (B) Ratio of perivascular area/vascular area. (C) Percentage of brain water content at 1 and 7 days after SAH. (D~E) Representative bands of Western blotting and corresponding densitometry analysis for expression levels of total Tau protein and phosphorylated Tau protein (PHF-1) in the dorsal forebrain at 7 days post SAH. Graphs are the summary of results from three independent experiments. Data shown as mean±SEM from 4 mice per group. The statistical analysis was performed by Student's t-test. ^*p<0.05, ^**p<0.01.