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. 2019 Feb 28;28(1):119–129. doi: 10.5607/en.2019.28.1.119

Table 2. Primary antibodies used in this case for diagnosis.

Antibodies Dilution Company Findings in this case
GFAP 1:300 DAKO, Glostrup, Denmark + in reactive astrocytes
NeuN 1:500 Millipore, Temecula, USA + in neurons
Neurofilament (NF) 1:2000 DAKO, Glostrup, Denmark + in axons and axonal spheroids
Phosphorylated NF 1:10,000 Millipore, Temecula, USA + in axons and axonal spheroids
Synaptophysin 1:100 Novocastra, Newcastle, UK + in gray matter
CD163 1:200 ABCAM, Bristol, UK + in pigmented microglia
CD68 1:2000 DAKO, Glostrup, Denmark + in a few of the pigmented microglia
α-synuclein 1:200 ABCAM, Bristol, UK Negative in entire brain
β-amyloid 1:500 Covance, Dallas, USA Negative in entire brain
3 repeat (3R) tau 1:100 Millipore, Ontario, Canada Negative in entire brain
4 repeat (4R) tau 1:1000 Millipore, Ontario, Canada Negative in entire brain
p-Tau (AT8) 1:100 ThermoFisher,Waltham, USA Negative in entire brain
p-TDP43 1:1,000 Cosmobio, Tokyo, Japan Negative in entire brain

GFAP, Glial fibrillary acidic protein; NeuN, Neuronal nuclei; CD, Cluster of differentiation; p-Tau, phospho-Tau; p-TDP43, phosphorylated TAR DNA binding protein.