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. 2019 Feb 25;10:273. doi: 10.3389/fimmu.2019.00273

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Copyright © 2019 Blaurock-Möller, Gröger, Siwczak, Dinger, Schmerler, Mosig and Kiehntopf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence staining of the lipoprotein ApoB in the hepatic layer of the liver-on-chip model. (A) Immunofluorescence staining of apolipoprotein B (ApoB, yellow) in the hepatic layer. Nuclei are stained with DAPI (blue). Data of one exemplary experiment out of six independent experiments is shown. Scale bar: 50 μm. (B) Computational analyses of mean fluorescence intensities (MFI) of at least 20 regions of interest (ROI) per tested condition of the respective protein using random field analysis. Results of six independent experiments are shown. Control (18846, [16685–19585]), CAAP48 (54411, [51883–57389]), CAAP47 (52463, [44992–59897]), Scrambled peptide (14787, [13910–17440]).