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. 2019 Feb 25;10:273. doi: 10.3389/fimmu.2019.00273

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Copyright © 2019 Blaurock-Möller, Gröger, Siwczak, Dinger, Schmerler, Mosig and Kiehntopf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence staining of endothelial proteins. (A) Expression of VE-cadherin (yellow), ZO-1 (green) and F-actin (red) at the vascular layer of the liver-on-chip model. Representative results of six independent experiments are shown. Scale bars 20 μm (VE-cadherin), 30 μm (ZO-1), 50 μm (F-actin). (B) Computational analyses of MFI of at least 20 ROI per tested condition of the respective protein using random field analysis. Results of six independent experiments are shown. VE-cadherin/ZO-1/F-Actin: Control (16302, [15550–16643]/18705, [17519–21225]/45716, [44318–48369]), CAAP48 (10562, [9561–11287]/16884, [16771–17111]/35908, [32545–39327]), CAAP47 (16458, [15837–16471]/18108, [17377–19042]/39179, [35624–47113]), Scrambled peptide (16017, [15789–16706]/17194, [17085–18375]/40668, [39056–46013]).