Figure 5.

Histological or immunohistochemical examination of collagenase-injected acupoints. (A,B) H&E staining of collagenase- or saline-treated acupoints. Quantification of pink-stained area in normal or collagenase-treated skins (B). ^*p < 0.05 vs. Normal; n = 5 per group. (C,D) Immunohistochemistry for PGP9.5, a neuronal marker, in normal or collagenase-treated acupoints (C). Mean fluorescent intensity in normal or collagenase-treated skins (D). n = 5 per group. COLG, collagenase. Bar = 50 μm. (E) Diffusion of a mixed solution of toluidine blue dye and type I collagenase injected into acupoints. Yellow line = blue area stained with toluidine blue. Bar = 1 mm.