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. 2019 Feb 26;10:153. doi: 10.3389/fphys.2019.00153

FIGURE 5.

FIGURE 5

Elevated inflammation was associated with IRI-induced AKI severity and the AKI-to-CKD transition. (A) Immunohistochemical staining of Ly6g (a neutrophil marker) at day 3 and F4/80 (a macrophage marker) at day 28 post ischemia in kidneys subjected to different durations of ischemia (original magnification × 400; Scale bar, 20 μm). (B) Semiquantitative data of Ly6g and F4/80 staining in different groups of mice. (C) Expression of inflammatory mediators (MCP1, TNF-α, and IL-6) was measured by real-time PCR. (D) Immunohistochemical staining of Ki-67 (a marker of proliferation) at day 3 and day 28 post ischemia in kidneys subjected to different durations of ischemia (original magnification × 400; Scale bar, 20 μm). (E) Semiquantitative data of Ki-67 staining in different groups of mice. T, tubule; I, interstitium. Data are presented as the means ± SEM of four experiments. n = 6; ∗P < 0.05 vs. the control group. #P < 0.05 vs. the 20 min-ischemia group. ΦP < 0.05 vs. the 30 min-ischemia group.