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. 2019 Feb 26;10:153. doi: 10.3389/fphys.2019.00153

FIGURE 6.

FIGURE 6

Repeated episodes of moderate IRI accelerated kidney injury in mice. (A) HE and Masson’s trichrome staining as well as immunohistochemical staining of α-SMA and collagen I in kidneys at day 14 after the first renal ischemia attack are shown (original magnification × 400; Scale bar, 20 μm). (B) Tubulointerstitial fibrosis score of mouse kidneys at day 14 after the first renal ischemia attack. (C) Changes in Scr levels in mice at day 14. (D) Semiquantitative data of α-SMA and collagen I staining in different groups of mice. (E) Western blot analysis of α-SMA in the kidney tissue lysates of mice in each group. The densitometry analyses of Western blot are shown. (F) Effect of the frequency of ischemia attack on the mRNA expression of fibrosis markers in kidney tissue lysates. Data are presented as the means ± SEM of four experiments. n = 6; ∗P < 0.05 vs. the sham control group. #P < 0.05 vs. the sham/sham group. ΦP < 0.05 vs. the single-attack group.