FIGURE 4.

The g6pd5, g6pd6, and g6pd5/6 mutant affect the ROS levels under salt stress. 1-day-old seeds and 5-day-old seedlings were grown vertically on ½ MS agar plates supplemented with the 150 mM NaCl for 12 h. (A) The levels of H₂O₂ were measured using the H₂DCF-DA fluorochrome dyes in Arabidopsis seeds. Bar, 200 μm. (B) The levels of H₂O₂ were measured using the H₂DCF-DA fluorochrome dyes in Arabidopsis roots. Bar, 200 μm. (C,D) Quantification of the fluorescence in Arabidopsis seeds and roots under NaCl treatment. (E) 10-day-old seedlings were grown vertically on ½ MS agar plates supplemented with the 150 mM NaCl for 12 h. Data are mean ± SE of three independent experiments, bars with different letters are significantly different at the level of P < 0.05. The experiment was repeated three with similar results.