Table 2.

Selection of reporter tools to quantify metabolites.

Tool	Method	Description	Validated in zebrafish	Reference
Perceval	Fluorescent biosensor	Genetically encoded ratiometric fluorescent reporter for ATP/ADP ratios	No	Berg et al., 2009
GCaMP6s	Fluorescent biosensor	Genetically encoded intensiometric fluorescent reporter for calcium	Yes	Chen J. et al., 2017; Singh et al., 2017; Janjuha et al., 2018a
RoGFP2-Orp1	Fluorescent sensor	Genetically encoded ratiometric fluorescent reporter for H2O2 detection	Yes	Panieri et al., 2017
Grx1-RoGFP2	Fluorescent sensor	Genetically encoded ratiometric fluorescent reporter for GSH:GSSG redox potential	Yes	Panieri et al., 2017
Cyto-roGFP	Fluorescent biosensor	Genetically encoded ratiometric fluorescent reporter for Redox state (Cytosol)	No	Waypa et al., 2010
Matrix-roGFP	Fluorescent biosensor	Genetically encoded ratiometric fluorescent reporter for Redox state (Mitochondrial Matrix)	No	Waypa et al., 2010
GPD-roGFP	Fluorescent biosensor	Genetically encoded ratiometric fluorescent reporter for Redox state (Mitochondrial Innermembrane space)	No	Waypa et al., 2010
Pyronic	FRET biosensor	Genetically encoded ratiometric fluorescent reporter for pyruvate	No	San Martin et al., 2014
Laconic	FRET biosensor	Genetically encoded ratiometric fluorescent reporter for lactate	No	San Martin et al., 2013
pHRed	Fluorescent biosensor	Genetically encoded intensiometric fluorescent reporter for pH	No	Tantama et al., 2011
Peredox-mCherry	FRET biosensor	Genetically encoded intensiometric fluorescent reporter for NADH/NAD ratio	No	Hung et al., 2011
iNap1	Fluorescent biosensor	Genetically encoded ratiometric fluorescent reporter for NADPH	Yes	Zou et al., 2018
SoNar	Fluorescent biosensor	Genetically encoded fluorescent reporter for NADH	Yes	Zhao et al., 2016; Zou et al., 2018
HyPerRed	Fluorescent biosensor	Genetically encoded intensiometric fluorescent reporter for H2O2	Yes	Zou et al., 2018