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. 2019 Feb 25;10:213. doi: 10.3389/fpls.2019.00213

FIGURE 3.

FIGURE 3

Subcellular localization and transcriptional activation of CsMYB85. (A) Subcellular localization of CsMYB85 in tobacco leaf cells. 35S:GFP was localized in both the nucleus and cytoplasm of tobacco leaf cells. In contrast, CsMYB330:GFP was observed only in the nucleus. 4’,6-Diamidino-2-phenylindole (DAPI) signals were localized only in the nucleus. Merged images show GFP and DAPI colocalization. Bar = 20 μm. (B) Transcriptional activation of CsMYB85 in yeast cells. The full-length coding sequence of CsMYB85 was inserted into the pGBKT7 (BD) vector. The pGADT7 (AD) vector and either BD-CsMYB85 or BD were transformed into cells of the Y2HGold yeast strain. Yeast cells containing AD and BD vectors were used as negative controls. The yeast was grown on SD media lacking –Leu/–Trp (–L/–T) or –Leu/–Trp/–His (–L/–T/–H) for 3 days at 30°C. Results were obtained from three independent transformation experiments.