Figure 4.

Inhibition of proteasome activity decreases the recruitment of phospho-Syk at the center of the immune synapse. (A) Representative confocal images of control and MG-132 treated B cells activated on antigen coated coverslides for different time points. Fluorescence intensity distribution of Phalloidin (green) and pSyk (red) was 3D plotted and quantified (side graphs) across the cell (dashed white lines). Rectangle in graphs, shown below images represent the central synaptic area. Scale bar = 10 μm. (B) Quantification of pSyk at the center of the IS for both conditions and different time points. ^****p < 0.001 (>70 cells). 2-way ANOVA with Sidak's post-test. Mean with SEM bars are shown.